|
| Status |
Public on Feb 17, 2012 |
| Title |
Zebrafish Globin Locus (ChIP-seq) |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Genome-wide analysis of H3K4me3 modifications, Gata1 binding, and DNase I hypersensitivity sites in zebrafish adult red blood cells
|
| |
|
| Overall design |
Zebrafish red cells from 10 adults were isolated for each ChIP-seq reaction. The red cells were cross-linked with formaldehyde for 20 min. DNA fragements bound by specific proteins were enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Gata1 and H3K4me3 (Millipore Cat. No. 17-614) as previously described in Lee et al 2006.
|
| |
|
| Contributor(s) |
Ganis JJ, Hsia N, Trompouki E, de Jong JL, DiBiase A, Jia Z, Sabo PJ, Weaver M, Sandstrom R, Stamatoyannopoulos JA, Zhou Y, Zon LI |
| Citation(s) |
22537494 |
| |
| Submission date |
Feb 16, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony Dominc DiBiase |
| E-mail(s) |
adibiase@enders.tch.harvard.edu
|
| Organization name |
Children's Hospital Boston
|
| Department |
Hem/Onc
|
| Lab |
Zon Lab
|
| Street address |
KFRB, 1 Blackfan St, Suite 8216
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9319 |
Illumina Genome Analyzer II (Danio rerio) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| SRA |
SRP010971 |
| BioProject |
PRJNA155707 |
Less...
More...