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Status |
Public on Aug 01, 2015 |
Title |
Expression data from cyclohexamide treated Human Foreskin Fibroblasts (HFFs) infected with type I Toxoplasma gondii and stimulated with interferon gamma. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Pre-infection with Toxoplasma parasites inhibits induction of an interferon gamma responsive gene expression program. In this study we aimed to determine what role host negative regulatory proteins that are induced by Toxoplasma infection, such as SOCS-1, have in this inhibition. We used Affymetrix microarrays to analyze host cell transcription after pre-treatment with the protein synthesis inhibitor cyclohexamide (CHX), Toxoplasma infection, and subsequent interferon-gamma stimulation.
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Overall design |
Human foreskin fibroblasts (HFFs) were left untreated or treated with CHX for 40min. Cells were infected, or not, with type I (RH) Toxoplasma parasites, for one hour, and subsequently stimulated with human IFN-γ for one hour. Total RNA was isolated and hybridized to Affymetrix Human U133A 2.0 gene chips.
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Contributor(s) |
Saeij JP, Rosowski EE |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Jan 06, 2012 |
Last update date |
Dec 06, 2018 |
Contact name |
Emily E Rosowski |
E-mail(s) |
rosowski@mit.edu
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Organization name |
MIT
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Department |
Biology
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Lab |
Saeij
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Street address |
77 Massachusetts Ave, 68-258d
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
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Platforms (1) |
GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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Samples (8)
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GSM857491 |
HFF-CHX,uninfect,IFNY |
GSM857492 |
HFF-untreat,RH,unstim |
GSM857493 |
HFF-untreat,RH,IFNY |
GSM857494 |
HFF-CHX,RH,unstim |
GSM857495 |
HFF-CHX,RH,IFNY |
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Relations |
BioProject |
PRJNA150163 |
Supplementary file |
Size |
Download |
File type/resource |
GSE34916_RAW.tar |
17.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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