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Series GSE2770 Query DataSets for GSE2770
Status Public on Sep 01, 2005
Title Transcriptional profiles of Th cells induced to polarize to Th1 or Th2 direction in the presence or absence of TGFbeta
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Th1 and Th2 cells arise from a common precursor cell in response to triggering through the TCR and cytokine receptors for IL-12 or IL-4. This leads to activation of complex signaling pathways, which are not known in detail. Disturbances in the balance between type 1 and type 2 responses can lead to certain immune-mediated diseases. Thus, it is important to understand how Th1 and Th2 cells are generated. To clarify the mechanisms as to how IL-12 and IL-4 induce Th1 and Th2 differentiation and how TGF-beta can inhibit this process, we have used oligonucleotide arrays to examine the early polarization of Th1 and Th2 cells in the presence and absence of TGF-beta after 0, 2, 6 and 48 hours of polarization.
Keywords: time course, differentiation
 
Overall design This study includes altoghether 34 samples. Six different types of treatments (Thp, Th0, Th1, Th2, Th1+TGFbeta, Th2+TGFbeta) were studied at 4 time points (0, 2, 6 and 48h). There are two biological replicates, for both time series, consisting of pooled samples derived from different individuals. The early time points (0, 2 and 6h) and late time point (0 and 48h) were done in separate experiments (cell from different individuals), because of the limited number of the cells obtained from each the cord blood.
 
Contributor(s) Lund RJ, Aittokallio T, Nevalainen O, Lahesmaa R, Kainonen E, Lahesmaa A, Dixon C, Ahlfors H, Melin O, Miller M
Citation(s) 14607935
Submission date Jun 06, 2005
Last update date Dec 13, 2018
Contact name Riikka Johanna Lund
E-mail(s) riikka.lund@utu.fi
Organization name University of Turku and Åbo Akademi University
Department Turku Centre for Biotechnology
Lab Molecular Immunology Group
Street address Tykistökatu 6 A
City Turku
ZIP/Postal code FIN-20521
Country Finland
 
Platforms (3)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
GPL8300 [HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array
Samples (102)
GSM60348 Th cells_no treatment_0h_replicate 1a (U95Av2)
GSM60349 Th cells_antiCD3+antiCD28+IL4+TGFbeta_2h_replicate 1a (U95Av2)
GSM60350 Th cells_antiCD3+antiCD28+IL4+TGFbeta_6h_replicate 1a (U95Av2)
Relations
BioProject PRJNA92301

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE2770_RAW.tar 302.4 Mb (http)(custom) TAR (of CEL)

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