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Status |
Public on Mar 07, 2012 |
Title |
Tiling array hybridizations of genomic DNA from Bacillus subtilis 168. |
Organism |
Bacillus subtilis subsp. subtilis str. 168 |
Experiment type |
Other
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Summary |
Genomic DNA prepared from B. subtilis 168 cells grown to stationary phase was hybridized to tiling arrays. The data are used in transcriptome studies to compute expression intensities from raw intensity data using a model of shift and drift and correcting for probe affinity variations as described in (Nicolas et al., 2009, Bioinformatics 25, 2341-2347).
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Overall design |
B. subtilis 168 was grown in LB medium to stationary phase. Genomic DNA was prepared from four independent cultures. After sonication, DNA was labeled with Cy3 and hybridized to tiling arrays.
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Contributor(s) |
Mäder U, Dervyn E, Nicolas P |
Citation(s) |
22383849 |
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Submission date |
Feb 18, 2011 |
Last update date |
Apr 07, 2014 |
Contact name |
Ulrike Mäder |
E-mail(s) |
ulrike.maeder@uni-greifswald.de
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Organization name |
University Medicine Greifswald
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Department |
Functional Genomics
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Street address |
F.-L.-Jahn-Str. 15A
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City |
Greifswald |
ZIP/Postal code |
D-17489 |
Country |
Germany |
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Platforms (1) |
GPL13168 |
BaSysBio Bacillus subtilis T2 385K array [090325_CBS_DTU_Bsub_T2] |
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Samples (4)
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Relations |
BioProject |
PRJNA137247 |