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| Status |
Public on Apr 17, 2024 |
| Title |
Effects of strigolactones on tomato leaf transcriptome under irrigated and repeated water stress conditions |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In response to environmental stressors, plants exhibit a phenomenon known as stress memory, whereby a prior stress episode influences their subsequent responses. This suggests the existence of a molecular memory mechanism that allows plants to adapt to recurring stress events. Previous work has highlighted the contribution of the phytohormone strigolactones to drought stress and memory thereof (10.1007/s00425-015-2266-8; 10.1111/nph.14190; 10.1111/pce.13758; 10.1093/pcp/pcac058). Additionally, there are sparse indications that especially (but not only) in Solanaceous plants, they may have a significant effect on reproduction (reviewed by 10.1111/pce.14461). However, the molecular underpinnings of both phenomena have not been researched yet. This study investigated the positioning of strigolactones to the tomato flowering network, and their contribution to drought memory.
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| Overall design |
We deep-sequenced total RNA from leaves of 3-week-old wt (M82 genotype) and strigolactone-depleted plants showing 70-80% reduction of strigolactone content in root tissues and exudates (SL-; line 6936 silenced in the CCD7 biosynthetic gene, kind gift by Dr. H. J. Klee, University of Florida, ). A total of 21 samples are included in the dataset, corresponding to 7 different conditions/treatments/genotypes each coming in 3 biological replicates; each biological replicate consisted of at least 3 fully expanded leaves (one per plant) from 5 plants per condition/treatment/genotype. The 7 kinds of samples are leaves from plants under: unstressed, irrigated conditions (control samples, both wt and SL- genotype - WT_WW and CC_WW); irrigated conditions after 1 drought episode (recovery 1, both for wt and SL- genotype - WT_S1 and CC_S1); irrigated conditions after 2 drought episodes ( (recovery 2, both for wt and SL- genotype - WT_S2 and CC_S2); unstressed, irrigated conditions but treated 24 h earlier with the synthetic strigolactone analogue GR245DS (wt only - WTTR24).
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| Contributor(s) |
Visentin I, Russo G, Capezzali C, Korwin Krukowski P, Campo E, Schubert A, Cardinale F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 16, 2024 |
| Last update date |
Apr 17, 2024 |
| Contact name |
IVAN VISENTIN |
| E-mail(s) |
plantstresslab2024@gmail.com
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| Phone |
+390116708805
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| Organization name |
University of Turin
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| Department |
DISAFA
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| Street address |
Largo Braccini 2
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| City |
Grugliasco |
| State/province |
Seleziona uno Stato |
| ZIP/Postal code |
10095 |
| Country |
Italy |
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| Platforms (1) |
| GPL27957 |
Illumina NovaSeq 6000 (Solanum lycopersicum) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA1100914 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE264066_genes.readcount.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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