|
| Status |
Public on Dec 31, 2014 |
| Title |
Somatic activity of the piRNA pathway in the mouse |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The piRNA pathway is necessary for the suppression of retrotransposon activity in germ cells and the maintenance of germ cell populations, but the existence of the pathway in adult somatic cells has not been demonstrated. This study investigates the activity of the piRNA pathway in somatic cells of the adult mouse. We find that the PIWI protein MIWI2 is expressed in a variety of somatic tissues. Immunoprecipitation of MIWI2 from extracts of spleen cells and Friend virus-transformed mouse erythroblasts (MEL cells) identifies abundant piRNAs encoded by multiple intergenic clusters. In comparison to MIWI2-bound testis piRNAs, the somatic piRNAs are slightly smaller, and markedly deficient in repeat-derived sequences, but they have the same preference for a uracil at the 5’ end; “ping-pong” amplification is absent.
|
| |
|
| Overall design |
Determination of MIWI2-bound piRNAs in mouse erythropoietic cells
|
| |
|
| Contributor(s) |
Boffelli D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 21, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Dario Boffelli |
| E-mail(s) |
dario.boffelli@cshs.org
|
| Organization name |
Cedars Sinai
|
| Department |
Pediatrics
|
| Lab |
Boffelli
|
| Street address |
700 N San Vicente Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90069 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| SRA |
SRP004912 |
| BioProject |
PRJNA135083 |
