 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 10, 2011 |
| Title |
Identification of DNA Methylation Markers for Lineage Commitment of in vitro Hepatogenesis [mRNA profiling] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Hepatocytes that have differentiated from human embryonic stem cells have great potential for the treatment of liver disease as well as for drug testing. Moreover, in vitro hepatogenesis is a powerful model system for studying the molecular mechanisms underlying liver development. DNA methylation is an important epigenetic mechanism that influences differential gene expression during embryonic development. We profiled gene expression and DNA methylation of three cell states of in vitro hepatogenesis—human embryonic stem cells, endoderm progenitors, and mature hepatocytes—using microarray analysis. Among 525 state-specific expressed genes, 67 showed significant negative correlation between gene expression and DNA methylation. State-specific expression and methylation of target genes were validated by quantitative reverse transcription–PCR and pyrosequencing, respectively. To elucidate genome-scale methylation changes beyond the promoter, we also performed high-throughput sequencing of methylated DNA captured by MBD2 protein [see SRA link below]. We found dynamic methylation changes in intergenic regions of the human genome during differentiation. Conclusion: This study provides valuable methylation markers for the lineage commitment of in vitro hepatogenesis and should help elucidate the molecular mechanisms underlying stem cell differentiation and liver development.
|
| |
|
| Overall design |
ES, EP, and MH expression assays were run in quadruplicate. RNA isolated from the cell line at the indicated time points was used for gene expression analysis using the Human-6 Whole-Genome Expression BeadChip (Illumina, San Diego, CA), which generates expression profiles for more than 46,000 human transcripts. Biotin-labeled cRNA was produced using a linear amplification kit (Ambion, Austin, TX) using 300 ng of quality-checked total RNA as input. Chip hybridizations, washing, Cy3-streptavidin staining, and scanning were performed on a BeadArray Reader (Illumina) platform using reagents and following protocols supplied by the manufacturer. The detection score was used to determine expression
|
| |
|
| Contributor(s) |
Kim M, Kim Y |
| Citation(s) |
21505074 |
| Submission date |
Nov 01, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Mirang Kim |
| E-mail(s) |
mirang@kribb.re.kr
|
| Phone |
82-42-879-8124
|
| Fax |
82-42-879-8119
|
| Organization name |
KRIBB
|
| Department |
human genome center
|
| Lab |
human genomics
|
| Street address |
52 Eoeun-dong
|
| City |
Daejeon |
| ZIP/Postal code |
305-333 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE25048 |
Identification of DNA Methylation Markers for Lineage Commitment of in vitro Hepatogenesis |
|
| Relations |
| BioProject |
PRJNA142755 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25046_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE25046_non-normalized_data.txt.gz |
4.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...