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| Status |
Public on Sep 28, 2010 |
| Title |
Expression data from peptidoglycan stimulated CD11b+ cells from NOD and NOD.Idd3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
CD11b+ CD11c- cells were isolated by cell sorting from the spleens of NOD and NOD.Idd3 mice. Cells were then either unstimulated or stimulated with peptidoglycan (5ug/ml) for 4 hours at 37 degrees prior to RNA extraction. Peptidoglycan was chosen as a stimulus due to its know ability to stimulate CD11b+ cells through Toll-like receptor 2 and our previous data showing biological differences between NOD and NOD.Idd3-derived CD11b+CD11c- cells after stimulation with peptidoglycan.
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| Overall design |
CD11b+ cells from NOD and NOD.Idd3 were either unstimulated or stimulated with peptidoglycan(PGN) for 4 hours at 37 degerees prior to RNA extraction.
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| Contributor(s) |
Liu SM, Anderson AC, Kuchroo VK |
| Citation(s) |
22019586 |
| |
| Submission date |
Sep 24, 2010 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Vijay Kuchroo |
| E-mail(s) |
vkuchroo@rics.bwh.harvard.edu
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| Organization name |
Harvard Medical School
|
| Street address |
77 Avenue Louis Pasteur, HIM 785
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM600311 |
NOD CD11b+ cells unstimulated, biological rep1 |
| GSM600312 |
NOD CD11b+ cells unstimulated, biological rep2 |
| GSM600313 |
NOD CD11b+ cells PGN stimulated, biological rep1 |
| GSM600314 |
NOD CD11b+ cells PGN stimulated, biological rep2 |
| GSM600315 |
Idd3 CD11b+ cells unstimulated, biological rep1 |
| GSM600316 |
Idd3 CD11b+ cells unstimulated, biological rep2 |
| GSM600317 |
Idd3 CD11b+ cells PGN stimulated, biological rep1 |
| GSM600318 |
Idd3 CD11b+ cells PGN stimulated, biological rep2 |
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| Relations |
| BioProject |
PRJNA132799 |
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