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| Status |
Public on Feb 08, 2011 |
| Title |
Mapping of H3K4 and H3K27 trimethylation in zebrafish cells (ChIP-chip) |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The genomic distribution of post-translationally modified histones in the zebrafish (Danio rerio) genome remain largely unraveled. The objective of this study was to (1) design a microarray covering all zebrafish gene promoters and 5' end of coding regions, and (ii) using these arrrays, map the genome-wide distribution of trimethylated H3K4 and H3K27 in the mbryo derived ZF4 cell line. Specifically, we determined teh enrichment profile of H3K4me3, H3K27me3 and of both (co-enriched) modifications in ZF4 cells. We determined average enrichment profiles through metagene analysis, functional terms enriched among genes marked by either modification, and pathway analysis.
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| Overall design |
ChIP-chip experiments were performed from duplicate cultures
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| Contributor(s) |
Collas P, Reiner AH, Lindeman L |
| Citation(s) |
21187971 |
| |
| Submission date |
Aug 18, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Philippe Collas |
| Organization name |
University of Oslo
|
| Department |
Institute of Basic Medical Sciences
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| Street address |
PO Box 1112 Blindern
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| City |
Oslo |
| ZIP/Postal code |
0317 |
| Country |
Norway |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE23872 |
Mapping of H3K4 and H3K27 trimethylation in zebrafish cells |
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| Relations |
| BioProject |
PRJNA133135 |
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