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| Status |
Public on Apr 01, 2011 |
| Title |
IGROV1 gene expression analysis after in vivo locoregional treatment with CpG-ODN |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Toll-like receptor 9 synthetic agonist oligodeoxynucleotides expressing CpG motifs are currently evaluated for their anti-tumor activity, mainly in association with DNA-damaging drugs. Microarray expression analyses of genes implicates in DNA repair on tumor cells from mice treated with CpG-OD, revealed a down-regulation in tumor cells. These findings provide the first time evidence that immune cells upon TLR9 engagement can sensitize cancer cells to DNA damaging chemotherapy.
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| Overall design |
IGROV-1 human ovarian carcinoma cells were adapted to growth intraperitoneally (i.p.) and maintained by serial i.p. passages of ascitic cells into healthy mice as described. Mice were injected i.p. with 2.5 x 106 ascitic cells in 0.2 ml of saline and treated starting 10-11 days later, when mice showed evident and established ascites, with CpG-ODN [phosphorothioated ODN1826 (5’-TCCATGACGTTCCTGACGTT-3’), TriLink Biotechnologies (San Diego, CA, USA)]. delivered i.p. at a dose of 20 µg/mouse for 3 consecutive days or 1 time, control mice received saline (4 mice/group). 24 hours after the last treatment with saline or CpG-ODN, ascites-bearing mice were sacrificed by cervical dislocation. Tumor cells adhered to peritoneal wall were collected and extraction immediately frozen in liquid nitrogen until RNA extraction.
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| Contributor(s) |
De Cecco L, Balsari A, Sommariva M |
| Citation(s) |
21878529 |
| Submission date |
Aug 05, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
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| Organization name |
IRCSS Istituto Nazionale Tumori
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| Street address |
via Venezian 1
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| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (8)
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| GSM575255 |
Saline 1 (tumor cells grown in mice) |
| GSM575256 |
CpG-ODN 2 (tumor cells grown in mice) |
| GSM575257 |
CpG-ODN 4 (tumor cells grown in mice) |
| GSM575258 |
Saline 3 (tumor cells grown in mice) |
| GSM575259 |
CpG-ODN 3 (tumor cells grown in mice) |
| GSM575260 |
Saline 2 (tumor cells grown in mice) |
| GSM575261 |
Saline 4 (tumor cells grown in mice) |
| GSM575262 |
CpG-ODN 1 (tumor cells grown in mice) |
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| This SubSeries is part of SuperSeries: |
| GSE23491 |
Gene expression profile of IGROV1 cells after treatment of saline or CpG-ODN |
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| Relations |
| BioProject |
PRJNA133331 |
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