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| Status |
Public on Dec 05, 2023 |
| Title |
LUTI-dependent Downregulation of a Cell Cycle Transcription Factor is Key for Timely Meiotic Entry |
| Organism |
Saccharomyces cerevisiae SK1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNA-seq to monitor expression of early meiotic genes and expression of mitotic transcription factor SBF targets. Increased SBF activity was achieved through either overexpression of SBF unique subunit Swi4 , removal of LUTI-mediated regulation of Swi4 levels, or nuclear depeletion of negative regulator of SBF Whi5.
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| Overall design |
Cells were grown as described in Su et al., 2023. Samples collected at 2 hours in SPO media. 2-3 biological replicates included for each genotype.
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| Contributor(s) |
Su AJ, Yendluri SC, Unal E |
| Citation(s) |
38411169 |
| |
| Submission date |
Feb 23, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Elcin Unal |
| E-mail(s) |
elcin@berkeley.edu
|
| Organization name |
UC Berkeley
|
| Department |
MCB
|
| Lab |
Unal
|
| Street address |
1 Barker Hall
|
| City |
Berkeley |
| State/province |
California |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL33179 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae SK1) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA938113 |
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