|
| Status |
Public on Mar 06, 2024 |
| Title |
Identification of the mesenchymal signals regulating embryonic mammary gland development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The development of branched organs like mammary and salivary glands is regulated by tissue interactions between the epithelial and mesenchymal tissues. Mammary gland consists of a branched epithelial ductal network embedded in a mammary specific mesenchyme. In order to uncover the molecular mechanisms regulating embryonic mammary gland development, we compared the transcriptomes of mesenchymes from different development stages and tissues by RNA-sequencing
|
| |
|
| Overall design |
E13.5 submandibular gland mesenchyme, E13.5 mammary gland mesenchyme, E13.5 ventral skin mesenchyme, E16.5 mammary gland mesenchyme and E16.5 fat pad from C57Bl/6JOlaHsd embryos were manually microdissected from the tissues after enzymatic treatment (Dispase II and Pancreatic-Trypsin consecutively). The mesenchymes isolated from 2-3 embryos from the same litter were pooled together as one sample. Altogether, five biological replicates were collected from 3 different litters.
|
| |
|
| Contributor(s) |
Lan Q, Trela E, Mikkola ML |
| Citation(s) |
38441552 |
| |
| Submission date |
Feb 22, 2023 |
| Last update date |
Mar 06, 2024 |
| Contact name |
Marja Mikkola |
| E-mail(s) |
marja.mikkola@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Street address |
P.O.Box 56
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (25)
|
|
| Relations |
| BioProject |
PRJNA937680 |
Less...
More...