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| Status |
Public on Jun 13, 2010 |
| Title |
A revised map of the human progenitor hierarchy reveals origin of macrophages and dendritic cells in early lymphoid development |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The classical model of hematopoiesis posits the segregation of lymphoid and myeloid lineages as the earliest fate decision. The validity of this model has recently been questioned in the mouse, however little is known concerning lineage potential of human progenitors. Here we provide a comprehensive analysis of the human hematopoietic hierarchy by clonally mapping the developmental potential of 7 progenitor classes from neonatal cord blood and adult bone marrow. Human multi-lymphoid progenitors, identified as a distinct population of Thy1-/loCD45RA+ cells within the CD34+CD38- stem cell compartment, gave rise to all lymphoid cell types, as well as monocytes, macrophages, and dendritic cells, indicating that these myeloid lineages arise in early lymphoid lineage specification. Thus, as in the mouse, human hematopoiesis does not follow a rigid model of myeloid-lymphoid segregation.
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| Overall design |
Total RNA was extracted from 5 - 10,000 sorted cord blood progenitor cells to compare gene expression
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| Contributor(s) |
Doulatov S, Notta F, Eppert K, Nguyen L, Ohashi P, Dick JE |
| Citation(s) |
20543838 |
| Submission date |
May 24, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Kolja Eppert |
| Organization name |
University Health Network
|
| Street address |
101 College St., rm 8-301
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA127175 |
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