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| Status |
Public on Feb 22, 2023 |
| Title |
Effect of nuclear pore complex (NPC) blockage by WGA treatment on zygotic genome activation (ZGA) during zebrafish early development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function of nuclear pore complex (NPC) in the regulation of zygotic genome activation (ZGA), we microinjected medium dosage of WGA in zebrafish embryos at 1-cell stage to block NPC function. We then performed gene expression profiling analysis using data obtained from RNA-seq of control or WGA treated embryo at comparable developmental time point (4.3 hours post fertilization (hpf) ) or develomental stage (dome).
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for zebrafish control embryo at 4.3 hpf of dome stage and WGA treated embryos at 4.3 hpf (developmental time matched) or 5 hpf (dome-like stage).
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| Contributor(s) |
Meng A, Shen W |
| Citation(s) |
36493774 |
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| Submission date |
Sep 08, 2022 |
| Last update date |
Feb 22, 2023 |
| Contact name |
Weimin Shen |
| Organization name |
School of life science, Tsinghua University
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| Street address |
Tsinghuayuan park
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (3) |
| GSM6562794 |
zebrafish early embryos, wildtype, 4.3 hpf, dome stage |
| GSM6562795 |
zebrafish early embryos, WGA treated, 4.3 hpf |
| GSM6562796 |
zebrafish early embryos, WGA treated, 5 hpf, dome-like stage |
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| Relations |
| BioProject |
PRJNA878480 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212929_FPKM_WGA.txt.gz |
354.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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