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| Status |
Public on Jun 10, 2010 |
| Title |
Clinical implications of gene dosage and gene expression patterns in diploid breast carcinoma (transcriptomic profiling) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genomic and expression profiling using 38K BAC array-CGH and Illumina HT-12 beadchips were performed on 97 diploid invasive breast tumors to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels. Patient stratification was performed according to axillary lymph node status (node-negative, pN0; node-positive, pN1) and overall survival (>8-year survivors; breast cancer-specific mortality within 8 years of diagnosis). Array-CGH results was validated by FISH using tumors showing HER2/neu gene amplification and expression profiling was confirmed using qPCR for 16 transcripts.
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| Overall design |
Total RNA was isolated from fresh-frozen tissue samples
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| Contributor(s) |
Parris TZ, Danielsson A, Nemes S, Kovács A, Delle U, Fallenius G, Möllerström E, Karlsson P, Helou K |
| Citation(s) |
20551037, 28877888 |
| Submission date |
Feb 22, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Toshima Z. Parris |
| E-mail(s) |
toshima.parris@oncology.gu.se
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| Organization name |
University of Gothenburg
|
| Department |
Department of Oncology
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| Lab |
Sahlgrenska Cancer Center
|
| Street address |
Medicinaregatan 1G, 6th floor
|
| City |
Gothenburg |
| ZIP/Postal code |
41390 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (97)
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| This SubSeries is part of SuperSeries: |
| GSE20486 |
Clinical implications of gene dosage and gene expression patterns in diploid breast carcinoma |
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| Relations |
| BioProject |
PRJNA129555 |
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