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Series GSE199844 Query DataSets for GSE199844
Status Public on Nov 02, 2022
Title Brain region and sex specific transcriptome of peripubertal stress induced adult male mice showing escalated aggression and adult female mice with no signs of aggression.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to uncover the molecular basis of peripubertal stress induced aggressive behaviour and its sex differences focussing on brain region specific transcriptome profiling (RNA-seq) and validation by quantitative reverse transcription polymerase chain reaction (qRT–PCR)
Methods: Hypothalamus (Hypo) and Prefrontal cortex (PFC) mRNA profiles of 90-day-old control adult male and female and PPS exposed escalated aggressive adult male (SEagg) mice and non aggressive adult female (SNagg) were sequenced on Illumina HiSeq 2500, in 3 biological replicates.The FASTQ sequencing reads were adapter-trimmed along with a minimum length cut-off of 50 bases using Prinseq-lite. The reads were aligned to mouse genome assembly using TopHat (v.2.0.11) followed by reference-based assembly using Cufflinks (v.2.2.1). Then differentially expressing transcripts were identified using Cuffdiff (v.2.2.1). qRT–PCR validation was performed using SYBR Green assays.
Results:Hypo of SEagg males showed 49 differentially expressed genes (DEGs) amongst which 28 were down-regulated, 20 were up-regulated and 1 was expressed in SEagg males but not in control males. PFC of SEagg males showed 87 DEGs amongst which 57 were downregulated, 28 were upregulated and 2 were only expressed in SEagg males but not control males. SNagg females showed 684 DEGs in Hypo and 152 DEGs in PFC when compared to their respective control samples.
Conclusions: We provide the first prefrontal cortex and hypothalamus specific transcriptome profiles of peripubertal stress (PPS) exposed, Balb/c adult male mice exhibiting escalated aggression and Balb/c adult female mice showing no signs of aggression.
 
Overall design Brain mRNA profiles of peripubertal stress exposed adult male and female mice.
 
Contributor(s) Konar A, Bhambri A, Pillai B
Citation(s) 36226913
Submission date Mar 31, 2022
Last update date Nov 03, 2022
Contact name Arpita Konar
E-mail(s) arpita.konar@igib.in
Organization name CSIR-Institute of Genomics and Integrative Biology
Street address Mathura Road
City New Delhi
ZIP/Postal code 110025
Country India
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (24)
GSM5988419 Male PFC Experimental BiologicalReplicate1
GSM5988420 Male PFC Experimental BiologicalReplicate2
GSM5988421 Male PFC Experimental BiologicalReplicate3
Relations
BioProject PRJNA821699

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199844_Female_Control_Hypo.csv.gz 339.8 Kb (ftp)(http) CSV
GSE199844_Female_Control_PFC.csv.gz 344.9 Kb (ftp)(http) CSV
GSE199844_Female_SNagg_Hypo.csv.gz 334.3 Kb (ftp)(http) CSV
GSE199844_Female_SNagg_PFC.csv.gz 341.3 Kb (ftp)(http) CSV
GSE199844_Male_Control_Hypo.csv.gz 326.9 Kb (ftp)(http) CSV
GSE199844_Male_Control_PFC.csv.gz 321.6 Kb (ftp)(http) CSV
GSE199844_Male_SEagg_Hypo.csv.gz 324.4 Kb (ftp)(http) CSV
GSE199844_Male_SEagg_PFC.csv.gz 335.4 Kb (ftp)(http) CSV
GSE199844_RAW.tar 6.4 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file
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