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| Status |
Public on Jul 25, 2022 |
| Title |
Transcriptome analysis of wildtype elmo3+/+ and homozygous elmo3-/- knockout zebrafish larvae at 120 hpf through next generation RNA sequencing [dataset 3] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Elmo3 is one member of the Elmo protein family. In our study we addressed the question if the functions of Elmo1, Elmo2 and Elmo3 ware consistent or if they differ. With the CRISPR/Cas9 system we generated single knockout mutants of elmo1, elmo2 and elmo3 in the zebrafish. To assess the impact of the loss of one of the proteins on the transcriptome of a developing organism, we did RNA sequencing and transcriptome analysis with elmo1+/+ and elmo1-/-, elmo2+/+ and elmo2-/- and elmo3+/+ and elmo3-/- zebrafish larvae at 120 hours post fertilization. Here we provide the sequencing data of elmo3+/+ and elmo3-/-.
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| Overall design |
Total RNA profiles of wildtype and homozygous elmo3 knockout zebrafish larvae samples
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| Contributor(s) |
Boger M, Sticht C, Kroll J |
| Citation(s) |
35874819 |
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| Submission date |
Mar 03, 2022 |
| Last update date |
Jul 29, 2022 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
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| Department |
ZMF
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| Street address |
Theodor-Kutzer-Ufer
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| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE197827 |
Transcriptome analysis of WT and homozygous elmo knockout zebrafish larvae at 120 hpf through next generation RNA sequencing |
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| Relations |
| BioProject |
PRJNA812402 |
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