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| Status |
Public on May 12, 2023 |
| Title |
Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [ATAC-Seq] |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors’ specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR-Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted towards photoreceptors or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species.
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| Overall design |
Head of zebrafish embryos at 18 hpf were dissected and used for Tn5 tagmentation. Tails were used for genotyping.
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| Contributor(s) |
Buono L, Letelier J |
| Citation(s) |
37227126 |
| |
| Submission date |
Nov 28, 2021 |
| Last update date |
Aug 11, 2023 |
| Contact name |
Lorena Buono |
| E-mail(s) |
lor.buono@gmail.com
|
| Organization name |
IRCSS-SDN
|
| Street address |
via Emanuele Gianturco 32
|
| City |
Napoli |
| ZIP/Postal code |
80121 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE189739 |
Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network |
|
| Relations |
| BioProject |
PRJNA784297 |
| SRA |
SRP348278 |
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