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| Status |
Public on Feb 10, 2022 |
| Title |
Transcriptomic Characterization of Pancreatic Cancer-Associated Macrophage Polarization |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The pancreatic ductal adenocarcinoma (PDA) microenvironment is composed of a variety of cell types and marked by extensive fibrosis and inflammation. Tumor-associated macrophages (TAM) are abundant, and they are important mediators of disease progression and invasion. TAMs are polarized in situ to a tumor promoting and immunosuppressive phenotype via cytokine signaling and metabolic crosstalk from malignant epithelial cells and other components of the tumor microenvironment (TME). However, the specific distinguishing features and functions of TAMs remain poorly defined. Here, we generated tumor-educated macrophages (TEM) in vitro andanalyzed the transcriptome.
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| Overall design |
Three replicates each of six conditions were subjected to polyA+ RNA-seq. Bone marrow-derived macrophages (BMDMs) were treated with M-CSF, LPS, IL4, or conditioned media from Kras-Off ("noDOX", 3 days or 5 days) or Kras-On ("plusDOX") pacreatic adenocarcinoma (PDA) cells.
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| Contributor(s) |
Boyer SM, Lee H, Magnuson B, Halbrook CJ, Lyssiotis CA |
| Citation(s) |
35156921 |
| |
| Submission date |
Nov 22, 2021 |
| Last update date |
May 12, 2022 |
| Contact name |
Brian Magnuson |
| E-mail(s) |
bmagnuso@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1500 East Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA782640 |
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