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| Status |
Public on Aug 25, 2022 |
| Title |
PI3K Signaling Specifies Proximal-Distal Fate by Driving a Developmental Gene Regulatory Network in SOX9+ Lung Progenitors [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In order to understand the epigenetic mechanisms directing development and differentiation of SOX9+ lung epithelial progenitor cells (EPCs), paired RNA-Seq and ATAC-Seq was performed on SOX9+ EPCs sorted using flow cytometry from a Sox9-GFP reporter mouse.
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| Overall design |
GFP+ positive lung epithelium was isolated by flow cytometry by selecting GFP+, CD326+, CD31-, CD45-, 7-AAD-negative cells into cell culture media. Nuclei were isolated, the transposase reaction was performed with Nextera Tn5 transposase (Illumina), and PCR amplification was performed according to previously published protocols (Buenrostro et al. 2015a).
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| Contributor(s) |
Khattar D, Fernandes S, Snowball J, Guo M, Gillen M, Sinner D, Jain S, Zacharias W, Swarr DT |
| Citation(s) |
35976093 |
| |
| Submission date |
Nov 04, 2021 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Daniel Todd Swarr |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Division of Neonatology & Pulmonary Biology
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| Street address |
3333 Burnet Ave, ML 7009
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| City |
Cincinnati |
| State/province |
OH- OHIO |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| GSM5673223 |
E11.5 Sox9+ Progenitor Cells - Sample 1 [ATAC-seq] |
| GSM5673224 |
E11.5 Sox9+ Progenitor Cells - Sample 2 [ATAC-seq] |
| GSM5673225 |
E16.5 Sox9+ Progenitor Cells - Sample 1 [ATAC-seq] |
| GSM5673226 |
E16.5 Sox9+ Progenitor Cells - Sample 2 [ATAC-seq] |
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| This SubSeries is part of SuperSeries: |
| GSE188239 |
PI3K Signaling Specifies Proximal-Distal Fate by Driving a Developmental Gene Regulatory Network in SOX9+ Lung Progenitors |
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| Relations |
| BioProject |
PRJNA777957 |
| SRA |
SRP344645 |
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