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| Status |
Public on Aug 25, 2022 |
| Title |
PI3K Signaling Specifies Proximal-Distal Fate by Driving a Developmental Gene Regulatory Network in SOX9+ Lung Progenitors [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to understand the epigenetic mechanisms directing development and differentiation of SOX9+ lung epithelial progenitor cells (EPCs), paired RNA-Seq and ATAC-Seq was performed on SOX9+ EPCs sorted using flow cytometry from a Sox9-GFP reporter mouse.
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| Overall design |
GFP+ positive lung epithelium was isolated by flow cytometry by selecting GFP+, CD326+, CD31-, CD45-, 7-AAD-negative cells into Trizol. Total RNA was isolated using RNEasy Micro Kits, and poly A RNA was sequenced as described below.
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| Contributor(s) |
Khattar D, Fernandes S, Snowball J, Guo M, Gillen M, Sinner D, Jain S, Zacharias W, Swarr DT |
| Citation(s) |
35976093 |
| |
| Submission date |
Nov 04, 2021 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Daniel Todd Swarr |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Division of Neonatology & Pulmonary Biology
|
| Street address |
3333 Burnet Ave, ML 7009
|
| City |
Cincinnati |
| State/province |
OH- OHIO |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
| GSM5672721 |
E11.5 Sox9+ Progenitor Cells - Sample 1 [RNA-seq] |
| GSM5672722 |
E11.5 Sox9+ Progenitor Cells - Sample 2 [RNA-seq] |
| GSM5672723 |
E11.5 Sox9+ Progenitor Cells - Sample 3 [RNA-seq] |
| GSM5672724 |
E16.5 Sox9+ Progenitor Cells - Sample 1 [RNA-seq] |
| GSM5672725 |
E16.5 Sox9+ Progenitor Cells - Sample 2 [RNA-seq] |
| GSM5672726 |
E16.5 Sox9+ Progenitor Cells - Sample 3 [RNA-seq] |
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| This SubSeries is part of SuperSeries: |
| GSE188239 |
PI3K Signaling Specifies Proximal-Distal Fate by Driving a Developmental Gene Regulatory Network in SOX9+ Lung Progenitors |
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| Relations |
| BioProject |
PRJNA777948 |
| SRA |
SRP344638 |
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