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Series GSE18301 Query DataSets for GSE18301
Status Public on Nov 24, 2009
Title Transcriptional profiling of the rat adipose tissue response to TCDD
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Rodents exposed to the environmental contaminant, TCDD, suffer from a number of acute and chronic toxicities, including lethality and a wasting syndrome. Hypothesizing that the wasting syndrome may be caused by changes in adipose tissue -- either in its hormonal regulation or in homeostatic effects -- we profiled the transcriptional response of rat white adipose to TCDD. We employed two separate rat strains: the Long-Evans strain is sensitive to TCDD toxicities while the Han/Wistar strain is over four orders of magnitude more resistant. One day after TCDD exposure few genes were altered in either strain, but after four days a modest number of transcriptional alterations were observed. Strikingly, TCDD had far fewer effects than did a feed-restriction protocol intended to mimic the wasting syndrome itself. Notably several classic TCDD-responsive genes were modulated at all time-points, including Cyp1a1, Cyp1b1, and Nqo1. We therefore concluded that rat adipose tissue is unlikely to be the primary driver of the wasting syndrome, and that another tissue is likely involved.
 
Overall design Two strains, each with drug-treated vs. vehicle-control
 
Contributor(s) Boutros PC, Okey AB, Pohjanvirta R
Citation(s) 26232522
Submission date Sep 28, 2009
Last update date Aug 21, 2015
Contact name Paul C Boutros
E-mail(s) Paul.Boutros@utoronto.ca
Organization name Ontario Institute for Cancer Research
Street address 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL9199 Affymetrix GeneChip Rat Genome 230 2.0 Array (CDF: Rat2302_Rn_ENTREZG.cdf version 12.0.0)
Samples (28)
GSM456913 Adipose-Long-Evans-Day1-TCDD-rep1
GSM456914 Adipose-Long-Evans-Day1-TCDD-rep2
GSM456915 Adipose-Long-Evans-Day1-TCDD-rep3
Relations
BioProject PRJNA118031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18301_RAW.tar 1.5 Gb (http)(custom) TAR (of CEL, DAT)
Processed data included within Sample table
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