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| Status |
Public on Feb 28, 2010 |
| Title |
Expresson from human THP-1 stimulated with IFN-g and/or infected with TB |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of interacting effects of TB and IFN-g on gene expression regulation within human macrophage-like THP-1 cells
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| Overall design |
To investigate the effect TB infection has on THP-1 macrophage’s response to IFN-g post-infection, in multiple experiments, THP-1 cells were exposed to each of the following four conditions: not treated, IFN-γ treated, M. tuberculosis infected and M. tuberculosis infected then IFN-γ treated. Total cellular RNA was then extracted. Recovered RNAs were pre-qualified for reproducibility by comparing qRT-PCR results of IFIT-2 and IP10 between replicate experiments. Four most reproducible replicate experiments were then selected for genome-wide mRNA abundance assays using Affymetrix microarray-based technology.
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| Contributor(s) |
Pine R |
| Citation(s) |
23378427 |
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| Submission date |
Aug 03, 2009 |
| Last update date |
Nov 24, 2019 |
| Contact name |
Richard Pine |
| E-mail(s) |
pineri@umdnj.edu
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| Phone |
973-854-3320
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| Organization name |
UMDNJ-NJMS
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| Department |
PHRI/Medicine
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| Street address |
225 Warren St
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| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (16)
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| GSM435893 |
THP1, IFN-g treated, biological rep 1 |
| GSM435894 |
THP1, IFN-g treated, biological rep 2 |
| GSM435895 |
THP1, IFN-g treated, biological rep 3 |
| GSM435896 |
THP1, IFN-g treated, biological rep 4 |
| GSM435897 |
THP1, TB infected, biological rep 1 |
| GSM435898 |
THP1, TB infected, biological rep 2 |
| GSM435899 |
THP1, TB infected, biological rep 3 |
| GSM435900 |
THP1, TB infected, biological rep 4 |
| GSM435901 |
THP1, TB infected, IFN-g treated, biological rep 1 |
| GSM435902 |
THP1, TB infected, IFN-g treated, biological rep 2 |
| GSM435903 |
THP1, TB infected, IFN-g treated, biological rep 3 |
| GSM435904 |
THP1, TB infected, IFN-g treated, biological rep 4 |
| GSM435905 |
THP1, Untreated, biological rep 1 |
| GSM435906 |
THP1, Untreated, biological rep 2 |
| GSM435907 |
THP1, Untreated, biological rep 3 |
| GSM435908 |
THP1, Untreated, biological rep 4 |
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| Relations |
| BioProject |
PRJNA118703 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17477_RAW.tar |
52.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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