|
| Status |
Public on May 08, 2021 |
| Title |
Transcriptional response to overexpressing Vibrio Seventh Pandemic -II island encoded genes |
| Organism |
Vibrio cholerae O1 biovar El Tor str. N16961 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: to characterize the regulatory targets of an AraC-like transcriptional regulator (VC0513) encoded on the Vibrio Seventh Pandemic Island -II (VSP-II) in V. cholerae O1 El Tor N16961
Methods: RNA was isolated from a wild-type N16961 carrying an IPTG-inducible copy of vc0513, vc0515, or an empty vector control
Results: vc0513 induction significantly increased expression of other VSP-II encoded genes relative to the empty vector control
Conclusions: our study represents the first analysis of a transcriptional regulator encoded on the VSP-II island
|
| |
|
| Overall design |
Examination of transcript profiles of vc0513 or vc0515 expressing V. cholerae relative to an empty vector control
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| |
|
| Contributor(s) |
Murphy SG, Doerr T |
| Citation(s) |
34153031 |
| Submission date |
May 07, 2021 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Tobias Doerr |
| E-mail(s) |
tdoerr@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Microbiology
|
| Lab |
Doerr Lab
|
| Street address |
525 N Campus Rd
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL30088 |
Illumina HiSeq 2000 (Vibrio cholerae O1 biovar El Tor str. N16961) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA727981 |
| SRA |
SRP318857 |
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