 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 07, 2022 |
| Title |
Expression data of shDIDO1 and shCtrl HUVEC cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
DIDO1(Death inducer-obliterator) gene localizes in the nucleus and cytosol, which is required in the early steps during the tumor progression and metastasis.
We analyzed the GeneChip expression profiles of human umbilical vein endothelial cells transfected by RNAi lentiviral vectors, shDIDO1 cell line and shCtrl cell lines
|
| |
|
| Overall design |
Firstly, we identified the DEGs between HUVEC cell lines (GSM4742969-GSM4742972) and the K562-HUVEC co-cultivated cell lines (GSM4742973-GSM4742976) by Human GeneChip primeview (Affymetrix, 901838). Four biological repeats were taken.
DIDO1 was identified as a key candidate gene, so we construct DIDO1-RNAi vector and transfected HUVEC cell lines. The DEGs between shDIDO1 cell lines (GSM4742963-GSM4742965) and the controls (shCtrl) (GSM4742966-GSM4742968) were analyzed by GeneChip. Three biological repeats were taken.
|
| |
|
| Contributor(s) |
Shen Z, Cao H |
| Citation(s) |
35178295 |
| |
| Submission date |
Aug 23, 2020 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Shen Zhenglei |
| E-mail(s) |
szl1020@163.com
|
| Organization name |
The third Affiliated Hospital of Kunming Medical University
|
| Street address |
No.519, Kunzhou Road, Xishan District
|
| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650118 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (14)
|
| GSM4742966 |
shCtrl-biological,rep1 |
| GSM4742967 |
shCtrl-biological,rep2 |
| GSM4742968 |
shCtrl-biological,rep3 |
| GSM4742969 |
HUVEC-biological, rep1 |
| GSM4742970 |
HUVEC-biological, rep2 |
| GSM4742971 |
HUVEC-biological, rep3 |
| GSM4742972 |
HUVEC-biological, rep4 |
| GSM4742973 |
K562-biological, rep1 |
| GSM4742974 |
K562-biological, rep2 |
| GSM4742975 |
K562-biological, rep3 |
| GSM4742976 |
K562-biological, rep4 |
|
| Relations |
| BioProject |
PRJNA658815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156713_RAW.tar |
27.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...