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| Status |
Public on Feb 28, 2020 |
| Title |
gdT cells and adipocyte IL-17RC control fat innervation and thermogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The sympathetic nervous system innervates peripheral organs to regulate their function and maintain homeostasis, whereas target cells also produce neurotrophic factors to promote sympathetic innervation. The molecular basis of this bi-directional communication remains to be fully elucidated. We use thermogenic adipose tissue as a model system to show that T cells, specifically gdT cells, play a critical role in promoting sympathetic innervation, at least in part through driving TGFβ1 expression in parenchymal cells via IL-17 Receptor C. Adipose-specific ablation of IL-17 Receptor C reduces TGFβ1 expression in adipocytes, impairs local sympathetic innervation and causes obesity and other metabolic phenotypes consistent with defective thermogenesis; innervation can be fully rescued by restoring TGFβ1 expression. Ablating gdT cells and the IL-17 Receptor C signaling pathway also impairs sympathetic innervation in salivary glands and the lung. These findings demonstrate T cell/parenchymal cell coordination to regulate sympathetic innervation.
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| |
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| Overall design |
Gene expression profiles of brown adipose tissue from WT or AdIl17rc KO mice.
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| |
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| Contributor(s) |
Spiegelman B, Hu B |
| Citation(s) |
32076265 |
| |
| Submission date |
Jan 25, 2020 |
| Last update date |
Feb 28, 2020 |
| Contact name |
Spiegelman Lab |
| Organization name |
Dana Farber Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Bruce Spiegelman
|
| Street address |
360 Longwood Avenue, Longwood Center
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA603193 |
| SRA |
SRP244968 |
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