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Status |
Public on Feb 01, 2020 |
Title |
Transcription profiling of tomato plants overexpressing SlMYB14 |
Organism |
Solanum lycopersicum |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In order to investigate the possible molecular mechanisms of SlMYB14 function in plant growth and stress responses, we employed the RNA-seq approach to identify the genes with altered expression levels in SlMYB14 transgenic plants.
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Overall design |
RNA-sequencing was carried out using two independent transgenic lines (OE5, OE7). Total RNA was isolated with Trizol reagent (Invitrogen, USA) from the aerial part of the one-week-old seedlings of 35Spro:SlMYB14 (OE5 and OE7)and Zhongshu 4 (ZS4) plants grown in parallel under unstressed conditions. Materials from 10 plants of each genotype were pooled for RNA isolation.
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Contributor(s) |
Li Z, Yao Q |
Citation missing |
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Submission date |
Dec 26, 2019 |
Last update date |
Feb 01, 2020 |
Contact name |
zhenjun li |
E-mail(s) |
zhenjun_1234@aliyun.com
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Organization name |
Shanghai academy of agricultural sciences
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Street address |
Beidi rd 2901
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City |
Shanghai |
ZIP/Postal code |
201106 |
Country |
China |
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Platforms (1) |
GPL16345 |
Illumina HiSeq 2000 (Solanum lycopersicum) |
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Samples (6)
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Relations |
BioProject |
PRJNA597746 |
SRA |
SRP238819 |