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| Status |
Public on Aug 14, 2019 |
| Title |
Gene expression changes in Retinoic Acid-treated zebrafish Vagus motor neurons |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Weprofiled the transcriptomes of DMSO-treated (control) and Retinoic Acid-treated zebrafish Vagus motor neurons at 38 hours post fertilization to identifiy genes that are differentially expressed between these two treatments.
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| Overall design |
We obtained cells by dissecting the hindbrains of Tg(isl1:Kaede)-expressing embryos, dissociating to single cell suspension, and sorting for Kaede-positive cells by flow cytometry. We sequenced 3 replicates of 4500-10000 cells per condition.
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| Contributor(s) |
Isabella AJ, Moens CB |
| Citation(s) |
32302545 |
| |
| Submission date |
Aug 13, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Adam J Isabella |
| E-mail(s) |
aisabell@fredhutch.org
|
| Phone |
2066675697
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Street address |
1100 Fairview Ave N B2-123
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA560048 |
| SRA |
SRP218233 |
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