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| Status |
Public on Sep 03, 2019 |
| Title |
Transcriptomic profiling of vegfc(hy-/-);vegfd(-/-) mutant hearts reveals that vegfc/d signaling and lymphatics vasculature play a role in cardiac metabolism [fresh tissue] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Recent studies have suggested that promoting lymphangiogenesis enhances cardiac repair following injury, but it is unknown whether lymphangiogenesis is required for cardiac regeneration. Here, we describe the anatomical distribution, regulation and function of the cardiac lymphatic network in a highly regenerative zebrafish model system using transgenic reporter lines and loss-of-function approaches.To understand the transcriptional profile of the model, we performed RNA-seq on cardiac ventricles from control and mutant (vegfc(hy-/-);vegfd(-/-)) ventricles. Overall, vegfc(hy-/-;vegfd-/-) mutant ventricles were characterized by an up-regulation of pathways related to sphingolipid and phospholipid metabolism, translation, protein maturation and nonsense mediated decay compared to control hearts. Our findings suggest that lymphatics vasculature play a role in lipid transport and when lymphatic function is compromised in vegfch(y-/-;vegfd-/-) mutant, there are consequences to sphingolipid and phospholipid metabolism. Vegfc(hy-/-;vegfd-/-) mutant ventricles were not characterized by an enrichment of genes pathways implicated in pathological hypertrophy. This finding suggests that the absence of cardiac lymphatics induced a physiological rather than pathological cardiac hypertrophy.
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| Overall design |
Transcriptional analysis of hearts from 2 different zebrafish lines.
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| Contributor(s) |
Porrello ER, Sim CB |
| Citation(s) |
31452940 |
| |
| Submission date |
Jun 21, 2019 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Enzo Porrello |
| Organization name |
Murdoch Children's Research Institute
|
| Department |
Cell Biology
|
| Lab |
Cardiac Regeneration
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| Street address |
c/o Royal Children’s Hospital
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| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3051 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE133131 |
Transcriptomic profiling of vegfc(hy-/-);vegfd(-/-) mutant hearts reveals that vegfc/d signaling and lymphatics vasculature play a role in cardiac metabolism |
|
| Relations |
| BioProject |
PRJNA550048 |
| SRA |
SRP202196 |
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