 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 12, 2009 |
| Title |
MCF7 time-series upon RITA treatment. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Targeting “oncogene addiction” is a promising strategy for anti-cancer therapy. Here, we report a potent inhibition of crucial oncogenes by p53 upon reactivation with small molecule RITA in vitro and in vivo. RITA-activated p53 unleashes transcriptional repression of anti-apoptotic proteins Mcl-1, Bcl-2, MAP4, and survivin, blocks Akt pathway on several levels and downregulates c-Myc, cyclin E and B-catenin. p53 ablates c-Myc expression via several mechanisms at transcriptional and posttranscriptional level. We show that transrepression of oncogenes correlated with higher level of p53 bound to chromatin-bound p53 than transactivation of pro-apoptotic targets. Inhibition of oncogenes by p53 reduces the cell’s ability to buffer pro-apoptotic signals and elicits robust apoptosis. Our study highlights the role of transcriptional repression for p53-mediated tumor suppression.
Keywords: time course
|
| |
|
| Overall design |
Breast carcinoma cell-line MCF7 was treated with the small-molecule p53 activator RITA for 2h, 8h, 16h and 24h.
|
| |
|
| Contributor(s) |
Enge M, Gluch A |
| Citation(s) |
19411072 |
| Submission date |
Oct 21, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Martin Enge |
| E-mail(s) |
martin.enge@ki.se
|
| Organization name |
Karolinska Institute
|
| Department |
Dep of Oncology-Pathology
|
| Street address |
CCK, Z4
|
| City |
Stockholm |
| ZIP/Postal code |
S-171 76 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA109617 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13291_RAW.tar |
9.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...