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Status |
Public on Jan 24, 2018 |
Title |
RNA-seq analysis of HuH-7, NCI-H1299 and HEK-293T cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The diversity and complexity of the cancer transcriptome hold the potential to yield tumor-specific transcripts (TSTs) in cancer. We report a novel LIN28B variant, LIN28B-TST, which is specifically expressed in hepatocellular carcinoma (HCC) and many other cancer types. LIN28B-TST initiates from a de novo alternative transcription initiation site. We found that LIN28B-TST was expressed in HuH-7 HCC cells and NCI-H1299 lung cancer cells, while wild-type LIN28B (LIN28B-WT) was expressed in HEK-293T human embryo kidney cells.
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Overall design |
Celluar RNAs were generated by deep sequencing using Illumina Hiseq.
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Contributor(s) |
Huang S, Guo W |
Citation missing |
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Submission date |
Jan 23, 2018 |
Last update date |
Dec 23, 2018 |
Contact name |
Shenglin Huang |
Organization name |
Fudan University
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Street address |
270 Dongan
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City |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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Platforms (1) |
GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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Samples (3) |
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Relations |
BioProject |
PRJNA431305 |
SRA |
SRP131169 |