|
| Status |
Public on Feb 28, 2018 |
| Title |
The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila |
| Organism |
Tetrahymena thermophila |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We hypothesize that Ibd1 coordinates the recruitment of several chromatin remodeling complexes to promote transcription of highly expressed genes in T.thermophila. We developed a ChIP-seq protocol including a heuristic method (in preparation paper, Ponce & Saettone, et al., 2017) to call peaks present in genic and intergenic regions specifically. We used the obtained data from the 2 NGS biological replicas, each of which had an INPUT and an Immunoprecipitated (IP) sample.
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| |
|
| Overall design |
Examination of 2 biological replicates of Ibd1 (BRD-containing protein) during vegetative growth.
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| |
|
| Contributor(s) |
Fillingham J, Ponce M, Saettone A |
| Citation(s) |
29523178 |
| |
| Submission date |
Aug 30, 2017 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Alejandro Saettone |
| E-mail(s) |
alejandro.saettonechipana@sickkids.ca
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics & Genome Biology
|
| Lab |
Campos Lab
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G0A4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL23968 |
Illumina HiSeq 2500 (Tetrahymena thermophila) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA401656 |
| SRA |
SRP131320 |
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