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| Status |
Public on Oct 11, 2018 |
| Title |
The aryl hydrocarbon receptor pathway defines the time frame for restorative neurogenesis |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
In contrast to mammals, zebrafish has high capacity to regenerate tissue architecture and replace lost neurons after traumatic brain injury. The ependymoglia cells react to the injury and generate new neurons, which migrate to the lessoned site and contribute to the tissue restoration. Understanding molecular and cellular mechanisms underlying this regenerative neurogenesis is the crucial step towards achieving regenerative therapies in the mammalian brain. In order to identify molecular pathways governing this mode of restorative neurogenesis, we analysed changes in the transcriptome of ependymoglia cells and their progeny during regeneration process and identified Aryl hydrocarbon receptor (AhR) to be involved in regulation of ependymoglia differentiation towards post-mitotic neurons.
Our in vivo imaging analysis shows that the high AhR signalling promotes direct conversion of newly identified population of ependymoglia into the post-mitotic neurons. In contrast, decrease in AhR signalling promotes ependymoglial proliferation. Interestingly, we observed inactivation of the AhR signalling shortly after the injury and return to the basal levels 7 days post injury (dpi) suggesting the role of AhR in proper timing of the direct conversion of ependymoglia, ensuring the successful repair. Taken together, we identified the role of AhR signalling pathway in regulating differentiation of ependymoglia into the neurons and setting up the proper timing of restorative neurogenesis in zebrafish brain.
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| Overall design |
We performed gene expression microarray analysis from zebrafish from the dorsal subventricular zone and the dorsal parenchyma. We analysed different time points after stab-wound injury and tissues from control animals
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| Contributor(s) |
Di Giaimo R, Durovic T, Barquin P, Kociaj A, Lepko T, Aschenbroich S, Breunig CT, Irmler M, Cernilogar FM, Schotta G, Barbosa J, Trümbach D, Baumgart EV, Neuner AM, Beckers J, Wurst W, Stricker SH, Ninkovic J |
| Citation(s) |
30566853 |
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| Submission date |
Aug 09, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
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| Organization name |
Helmholtz Zentrum Muenchen
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| Department |
Institute of Experimental Genetics
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| Street address |
Ingolstaedter Landstr. 1
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| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL16933 |
[ZebGene-1_0-st] Zebrafish Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (26)
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| GSM2736042 |
Dorsal parenchyma, day 2 post lesion, biological replicate 1 |
| GSM2736043 |
Dorsal parenchyma, day 2 post lesion, biological replicate 2 |
| GSM2736044 |
Dorsal parenchyma, day 2 post lesion, biological replicate 3 |
| GSM2736045 |
Dorsal parenchyma, day 7 post lesion, biological replicate 1 |
| GSM2736046 |
Dorsal parenchyma, day 7 post lesion, biological replicate 2 |
| GSM2736047 |
Dorsal parenchyma, day 7 post lesion, biological replicate 3 |
| GSM2736048 |
Dorsal parenchyma, no lesion, biological replicate 1 |
| GSM2736049 |
Dorsal parenchyma, no lesion, biological replicate 2 |
| GSM2736050 |
Dorsal subventricular zone, day 2 post lesion, biological replicate 1 |
| GSM2736051 |
Dorsal subventricular zone, day 2 post lesion, biological replicate 2 |
| GSM2736052 |
Dorsal subventricular zone, day 2 post lesion, biological replicate 3 |
| GSM2736053 |
Dorsal subventricular zone, day 7 post lesion, biological replicate 1 |
| GSM2736054 |
Dorsal subventricular zone, day 7 post lesion, biological replicate 2 |
| GSM2736055 |
Dorsal subventricular zone, day 7 post lesion, biological replicate 3 |
| GSM2736056 |
Dorsal subventricular zone, no lesion, biological replicate 1 |
| GSM2736057 |
Dorsal subventricular zone, no lesion, biological replicate 2 |
| GSM2736058 |
Dorsal subventricular zone, no lesion, biological replicate 3 |
| GSM2736059 |
Medial neurogenic zone, day 2 post lesion, biological replicate 1 |
| GSM2736060 |
Medial neurogenic zone, day 2 post lesion, biological replicate 2 |
| GSM2736061 |
Medial neurogenic zone, day 2 post lesion, biological replicate 3 |
| GSM2736062 |
Medial neurogenic zone, day 2 post lesion, biological replicate 4 |
| GSM2736063 |
Medial neurogenic zone, day 7 post lesion, biological replicate 1 |
| GSM2736064 |
Medial neurogenic zone, day 7 post lesion, biological replicate 2 |
| GSM2736065 |
Medial neurogenic zone, day 7 post lesion, biological replicate 3 |
| GSM2736066 |
Medial neurogenic zone, no lesion, biological replicate 1 |
| GSM2736067 |
Medial neurogenic zone, no lesion, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA397660 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102400_RAW.tar |
119.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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