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| Status |
Public on Feb 22, 2018 |
| Title |
RNA-seq reveals abundant circRNA, lncRNA and mRNA in blood exosomes of patients with hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Exosomes are small membrane vesicles of endocytic origin secreted by most cells, and contain a wealthy cargo of protein and RNA species that can modulate recipient cells’ behaviors and may be used as biomarkers for diagnosis of human diseases. They have been found in blood and are valuable sources for biomarkers due to selective cargo loading and resemblance to their parental cells. The goal of this study is to identify circRNA, lncRNA and mRNA profiles in human blood by high-throughput RNA sequencing (RNA-seq). 1-4 ml plasma or serum were used to extract exosomal RNAs by exoRNeasy Serum/Plasma Maxi kit (Qiagen). The exosomal RNAs were further treated with DNAse I and subjected to ribosome minus low-input RNAseq library preparation. The libraries were sequenced by Illumina Hiseq platform.
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| Overall design |
Human blood exosomal RNAs were generated by deep sequencing using Illumina Hiseq.
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| Contributor(s) |
Wang L |
| Citation |
Li et.al. exoRBase: a database of circRNA, lncRNA and mRNA in human blood exosomes. Nucleic Acids Research, Volume 46, Issue D1, 4 January 2018
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| |
| Submission date |
Jun 19, 2017 |
| Last update date |
Oct 12, 2023 |
| Contact name |
Shengli Li |
| E-mail(s) |
lishenglibio@outlook.com
|
| Phone |
86-21-34777580
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| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
650 Xinsongjiang Road
|
| City |
Shanghai |
| ZIP/Postal code |
201620 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA390991 |
| SRA |
SRP109668 |
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