GEO DataSets

(Submitter supplied) Zur is a transcription factor that represses zinc starvation response genes under zinc-rich conditions. Our goal was to identify candidate Zur-regulated genes by looking for transcripts significantly upregulated in the absence of Zur.

Organism:

Vibrio cholerae O1 biovar El Tor str. N16961

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30088

6 Samples

Download data: CSV, TXT

(Submitter supplied) Purpose: to characterize the regulatory targets of an AraC-like transcriptional regulator (VC0513) encoded on the Vibrio Seventh Pandemic Island -II (VSP-II) in V. cholerae O1 El Tor N16961 Methods: RNA was isolated from a wild-type N16961 carrying an IPTG-inducible copy of vc0513, vc0515, or an empty vector control Results: vc0513 induction significantly increased expression of other VSP-II encoded genes relative to the empty vector control Conclusions: our study represents the first analysis of a transcriptional regulator encoded on the VSP-II island

Organism:

Vibrio cholerae O1 biovar El Tor str. N16961

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30088

9 Samples

Download data: TXT, XLSX

(Submitter supplied) Cholera is a diarrheal disease caused by Vibrio cholerae of serogroups O1 and O139 that affects impoverished populations worldwide. The histone-like nucleoid structuring protein (H-NS) is a global regulator of environmentally-regulated gene expression that plays a fundamental role in V. cholerae adaptation to disparate ecological niches. We used RNA-seq to characterize the hns transcriptome of El Tor biotype V. more...

Organism:

Vibrio cholerae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19265

8 Samples

Download data: TXT, XLS

(Submitter supplied) The Gram-negative bacterium Vibrio cholerae adapts to changes in environment by selectively producing the necessary machinery to uptake and metabolize available carbohydrates. The import of the six-carbon sugar fructose by the fructose-specific phosphoenolpyruvate (PEP) phosphotransferase system (PTS) is of particular interest because of its putative connection to biofilms, which have been shown to promote host colonization and cholera pathogenesis. more...

Organism:

Vibrio cholerae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27062

8 Samples

Download data: TXT

(Submitter supplied) Using a combination of ChIP-seq and RNA-seq we show that ToxT regulates expression of the small RNA TarB. TarB modulates the expression level of the transcriptioanl repressor VC0177. VC0177 controls expression of several genes including VC0179.

Organism:

Vibrio cholerae O1 str. C6706

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL15333 GPL15332

23 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of an El Tor biotype crp mutant The virulent V. cholerae El Tor Ogawa strain C7258 (Peru isolate 1991) and an isogenic deletion mutant (WL7258) lacking DNA sequences encoding the cAMP receptor protein were grown in LB medium to optical density at 600 nm of 1.5. The cultures were chilled in ice, cells quickly collected by centrifugation and total RNA imediately extracted. RNAwas extracted and purified using the Trizol plus RNA purification system (Invitrogen) followed RNEasy miniElute cleanup (Qiagen). more...

Organism:

Vibrio cholerae

Type:

Expression profiling by array

Platform:

GPL5085

4 Samples

Download data: CSV, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Vibrio cholerae O1 str. C6706

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15333

7 Samples

Download data: TXT

(Submitter supplied) The transcriptional factor ToxR initiates a virulence regulatory cascade required for V. cholerae to express critical host colonization factors and cause disease. Genome-wide expression studies suggest that ToxR regulates many genes important for V. cholerae pathogenesis, yet our knowledge of the direct regulon controlled by ToxR is limited to just four genes. Here, we determine ToxR’s genome-wide DNA-binding profile and show that ToxR is a global regulator of both progenitor genome-encoded genes and horizontally acquired islands encoding the majority of V. more...

Organism:

Vibrio cholerae O1 str. C6706

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15333

3 Samples

Download data: TXT

(Submitter supplied) The transcriptional factor ToxR initiates a virulence regulatory cascade required for V. cholerae to express critical host colonization factors and cause disease. Genome-wide expression studies suggest that ToxR regulates many genes important for V. cholerae pathogenesis, yet our knowledge of the direct regulon controlled by ToxR is limited to just four genes. Here, we determine ToxR’s genome-wide DNA-binding profile and show that ToxR is a global regulator of both progenitor genome-encoded genes and horizontally acquired islands encoding the majority of V. more...

Organism:

Vibrio cholerae O1 str. C6706

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15333

3 Samples

Download data: TXT

(Submitter supplied) The transcriptional factor ToxR initiates a virulence regulatory cascade required for V. cholerae to express critical host colonization factors and cause disease. Genome-wide expression studies suggest that ToxR regulates many genes important for V. cholerae pathogenesis, yet our knowledge of the direct regulon controlled by ToxR is limited to just four genes. Here, we determine ToxR’s genome-wide DNA-binding profile and show that ToxR is a global regulator of both progenitor genome-encoded genes and horizontally acquired islands encoding the majority of V. more...

Organism:

Vibrio cholerae O1 str. C6706

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15333

3 Samples

Download data: TXT