GEO DataSets

(Submitter supplied) Clostridium botulinum ATCC 3502 was grown in continuous culture at 39°C, subjected to heat shock at 45°C, and then continuously grown at 45°C. The goal was to determine the impact of stressful temperature to the whole genome expression profile and identify stress adaptation mechanisms.

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Expression profiling by array

Platform:

GPL11441

28 Samples

Download data: GPR

(Submitter supplied) Profound understanding of the mechanisms foodborne pathogenic bacteria utilize in adaptation to the environmental stress they encounter during food processing and storage is of paramount importance in design of control measures. Chill temperature is a central control measure applied in minimally processed foods; however, data on the mechanisms the foodborne pathogen Clostridium botulinum activates upon cold stress are scarce. more...

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Expression profiling by array

Platform:

GPL11441

6 Samples

Download data: TXT

(Submitter supplied) The two-component system CBO0366/CBO0365 was recently demonstrated to have a role in cold tolerance of Group I Clostridium botulinum ATCC 3502. The mechanisms under its control, ultimately resulting in increased sensitivity to low temperature, are unknown. A transcriptomic analysis with DNA microarrays was performed to identify the differences in global gene expression patterns of the wild-type ATCC 3502 and a derivative mutant with insertionally inactivated cbo0365 at 37 °C and 15 °C. more...

Organism:

Clostridium botulinum A str. ATCC 3502

Type:

Expression profiling by array

Platform:

GPL11441

6 Samples

Download data: GPR

(Submitter supplied) The members of the genus Clostridium, such as the other spore-forming anaerobic bacteria, have a complex and strictly regulated life-cycle but very little is known about genetic pathways involved at different stages. Clostridium sporogenes, Gram positive bacterium usually involved in food spoilage and frequently isolated from late blowed cheese is genetically indistinguishable from proteolytic Clostridium botulinum and is the non-neurotoxigenic counterpart often used as exemplar for the toxic subtypes. more...

Organism:

Clostridium botulinum A str. ATCC 3502; Clostridium sporogenes

Type:

Expression profiling by array

Platform:

GPL15677

12 Samples

Download data: TXT

(Submitter supplied) C. botulinum UMASS Type A strain was compared to the ATCC 3502 Type A reference strain.

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Genome variation profiling by genome tiling array

Platform:

GPL6392

1 Sample

Download data: GFF, PAIR

(Submitter supplied) Strains were differentiated on the basis of hybridization to probes representing strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes.

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Genome variation profiling by array

Platform:

GPL8538

27 Samples

Download data: TXT

(Submitter supplied) Strains were differentiated on the basis of hybridization to probes representing strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes.

Organism:

Clostridium butyricum; Clostridium argentinense; Clostridium botulinum; Clostridium sporogenes; Clostridium baratii; Clostridium botulinum A str. ATCC 3502; Clostridium perfringens

Type:

Genome variation profiling by array

Platform:

GPL8538

44 Samples

Download data: TXT

(Submitter supplied) Comparative genomic hybridization microarrays featuring overlapping probes spanning the entire C. botulinum type A1 strain ATCC 3502 genome were used to identify regions whose presence are variable among a diverse panel of type A C. botulinum strains.

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Genome variation profiling by genome tiling array

Platform:

GPL6392

10 Samples

Download data: GFF, PAIR

(Submitter supplied) Comparative genomic hybridizations were performed to compare bont/A1 strains with an A2-like toxin gene cluster organization to the genome sequenced strain, C. botulinum ATCC 3502. Keywords: comparative genomic hybridization

Organism:

Clostridium botulinum; Clostridium botulinum A str. ATCC 3502

Type:

Genome variation profiling by array

Platform:

GPL6392

7 Samples

Download data: GFF, PAIR

(Submitter supplied) Spots size: 25 microns Total number of spots: 94928 Number of spots replicates per oligo: 3 Probes corresponding to 3400 genomic CDSs of C. botulinum A ATCC 3502 were synthesized in three replicates randomly distributed on the chip. Protocol: Probes are directly synthesized on a 12k Combimatrix array by an in situ oligo synthesis system developed by Combimatrix Corporation (Mukilteo, USA) using the Combimatrix CustomArray synthesizer. more...

Organism:

Clostridium botulinum A str. ATCC 3502

1 Series

12 Samples

Download data

(Submitter supplied) Agilent 60mer

Organism:

Clostridium botulinum A str. ATCC 3502

3 Series

40 Samples

Download data

(Submitter supplied) Probes represent strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes. The Platform data table reflects a condensed representation of the array's replicate features. The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record. more...

Organism:

Clostridium botulinum A str. ATCC 3502

2 Series

54 Samples

Download data: TXT

(Submitter supplied) Oligonucleotide arrays were synthesized by NimbleGen Systems, Inc by maskless array synthesis. A custom comparative genomic hybridization design was used based on the C. botulinum ATCC 3502 genome sequence (GenBank accession number: AM412317). The NimbleGen design file (.ndf) linked as supplementary file.

Organism:

Clostridium botulinum A str. ATCC 3502

3 Series

15 Samples

Download data: NDF