Genome skimming corals for systematics and DNA barcodes - SRA

SRX22649220: Genome skimming corals for systematics and DNA barcodes
1 ILLUMINA (Illumina NovaSeq 6000) run: 4M spots, 1.2G bases, 371.2Mb downloads

Design: Library preparation was carried out in the Laboratories of Analytical Biology at the Smithsonian Institution using the NEBNext Ultra II FS DNA Library Prep Kit for inputs 100 ng with the following modifications: the reaction volume was reduced by half, the fragmentation/end prep incubation was conducted for 10 minutes (contemporary samples) or 2.5 minutes (historical samples), 5 l of iTru Y-yoke adaptor (Glenn et al., 2019) was used instead of NEBNext Adaptor, adaptor ligation time was 30 minutes, bead cleanups were performed with KAPA Pure Beads, iTru i5 and i7 indices (Glenn et al., 2019) were used, and 10 cycles of PCR enrichment were conducted. A negative control was included on each plate during library preparation to test for any potential contamination. All DNA libraries were quantified and assessed with a Qubit fluorometer High Sensitivity Assay and a Tapestation, and final pools were created for sequencing on an Illumina NovaSeq (150 bp paired-end (PE) reads).

Submitted by: Smithsonian Institution

Study: Skimming genomes for systematics and DNA barcodes of corals

PRJNA1043120 • SRP474401 • All experiments • All runs

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Project detaiing a genome skimming approach followed by bioinformatically pulling UCEs and exons from the low-coverage genome data.

Sample:

SAMN38332690 • SRS19645527 • All experiments • All runs

Organism: Aphanostichopathes sp.

Library:

Name: NF22002

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 4M spots, 1.2G bases, 371.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR26955455	4,045,520	1.2G	371.2Mb	2023-11-27

ID:: 30661948

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