Genome sequencing and assembly of Klebsiella pneumoniae - SRA

SRX8370305: Genome sequencing and assembly of Klebsiella pneumoniae
1 ILLUMINA (Illumina NovaSeq 6000) run: 2.4M spots, 715.9M bases, 224.6Mb downloads

Design: Genomic DNA was isolated from cultures grown in Lysogeny Broth overnight. DNA was extracted in 96-well format from 100 uL of sample using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform. Bead disruption was conducted on a TissueLyser II (20 Hz for 20 min) instrument in a 96 deep well plate in the presence of 200 uL phenol/chloroform. Genomic DNA was eluted in 90 ul water after magnetic bead cleanup. The resulting genomic DNA was quantified by Pico Green.

Submitted by: University of Maryland, School of Medicine

Study: Klebsiella pneumoniae Genome sequencing and assembly

PRJNA633565 • SRP262496 • All experiments • All runs

Sample:

SAMN14967672 • SRS6685590 • All experiments • All runs

Organism: Klebsiella variicola

Library:

Name: CRE184

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.4M spots, 715.9M bases, 224.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR11819343	2,370,635	715.9M	224.6Mb	2020-09-24

ID:: 10887502

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