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SRX912170: NGS Types
1 ION_TORRENT (Ion Torrent PGM) run: 362,887 spots, 44.3M bases, 32.8Mb downloads

Design: 10 types. PCR 1 with 10 bp tailed primers, 60 cycles. PCR 2 with adapter tailed primers, 40 cycles. DSS was 0.5:1 (beads:PCR) for uppercut and 0.8:1 for lowercut.
Submitted by: University of Guelph
Study: DNA barcoding old type specimens
PRJNA277350 • SRP055961 • All experiments • All runs
show Abstracthide Abstract
We use traditional Sanger and next-generation sequencing to obtain COI barcodes from Lepidoptera type specimens that range in age from decades to centuries old.
Sample: 164 bp type
SAMN03389411 • SRS868126 • All experiments • All runs
Organism: Axinoptera fasciata
Library:
Name: 164 bp
Instrument: Ion Torrent PGM
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Spot descriptor:
         5  forward

Runs: 1 run, 362,887 spots, 44.3M bases, 32.8Mb
Run# of Spots# of BasesSizePublished
SRR1867817362,88744.3M32.8Mb2015-09-15

ID:
1311113

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