GSM1625956: FRE-3 (miRNA); Physcomitrella patens; miRNA-Seq - SRA

SRX903105: GSM1625956: FRE-3 (miRNA); Physcomitrella patens; miRNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 11.4M spots, 559.5M bases, 398.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Genome-wide small RNAtranscriptome analysis during dehydration and recovery in gametophores of Physcomitrella patens

PRJNA277372 • SRP055886 • All experiments • All runs

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Small RNA transcript abundances were analyzed with samples taken from hydrated (HD), moderate dehydration (MDH), desiccated (SDH), partially recovered (PRE) and fully recovered (FRE) gametophores of P. patens by using next generation sequencing. Totally, 147 microRNAs were identified as differentially expressed miRNAs.The results provided insight for exploring the mechanisms of desiccation tolerance and their evolution. Overall design: Examination of miRNA transcript abundances in hydrated (HD), moderate dehydration (MDH), desiccated (SDH), partially recovered (PRE) and fully recovered (FRE) gametophores, for each treatment, two biological replicates were included.

Sample: FRE-3 (miRNA)

SAMN03389875 • SRS865577 • All experiments • All runs

Organism: Physcomitrella patens

Library:

Instrument: Illumina HiSeq 2000

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: Total RNA extracted from hydrated (HD), mild dehydrated (MDH), severe dehydrated (SDH), partially rehydrated (PRE) and fully rehydrated (FRE) gametophores of moss P. patens. Enrich 18-30nt RNA fragments; add 5' and 3' adaptors; cDNA sysnthesis by reverse trancription; PCR amplification using 5' and 3' adaptor primers. Qualified library for sequencing.

Experiment attributes:

GEO Accession: GSM1625956

Links:

External link: GEO Sample GSM1625956

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11.4M spots, 559.5M bases, 398.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1842138	11,418,109	559.5M	398.1Mb	2018-02-22

ID:: 1297303

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