U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ERX443237: Illumina HiSeq 2000 paired end sequencing; RNA-seq of Sphagnum subsecundum and S. cribrosum collected in the field and grown under common garden condition for 6 months
1 ILLUMINA (Illumina HiSeq 2000) run: 94.8M spots, 19G bases, 10.3Gb downloads

Design: RNA-seq of Sphagnum subsecundum and S. cribrosum collected in the field and grown under common garden condition for 6 months
Submitted by: Biology Department, Duke University
Study: RNA-seq of Sphagnum subsecundum and S. cribrosum collected in the field and grown under common garden condition for 6 months
show Abstracthide Abstract
In diploid organisms, selfing reduces the efficiency of selection in purging deleterious mutations from a population. This need not be the case for all organisms. Some plants, for example, undergo an extreme from of selfing known as intragametophytic selfing, which immediately exposes all recessive deleterious mutations in a parental genome to selective purging. Using the transcriptome data from S. subsecundum, S. cribrosum and 2 other previously published moss transcriptome, we ask how effectively deleterious mutations are purged from such plants. Specifically, we study genome-wide signatures of selective purging in the transcriptomes of four moss species that differ in their incidence of selfing and outcrossing.
Sample: E-MTAB-2482:WFJL
SAMEA2465268 • ERS435074 • All experiments • All runs
Library:
Name: WFJL
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: PAIRED
Construction protocol: total RNA extracted with Spectrum Plant Total RNA Kit (Sigma) TruSeq RNA sample prep kit used for library preparation
Spot descriptor:
forward101  reverse

Experiment attributes:
Experimental Factor: organism: Sphagnum cribrosum
Runs: 1 run, 94.8M spots, 19G bases, 10.3Gb
Run# of Spots# of BasesSizePublished
ERR47740494,818,99619G10.3Gb2014-06-30

ID:
898140

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...