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SRX997482: GSM1659655: PXO99A with dTal280 replicate 1; Oryza sativa Japonica Group; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 29.6M spots, 3G bases, 2.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Host transcriptional reprogramming in response to the rice bacterial blight pathogen Xanthomonas oryzae pv.oryzae constitutively expressing the X. oryzae pv. oryzicola TAL2a effector from the high copy vector pKEB31
show Abstracthide Abstract
We performed RNA-Seq of leaves of Oryza sativa L. ssp. japonica cv. Nipponbare 48 hours after inoculation with  Xanthomonas oryzae pv. oryzae strain PXO99A heterologously expressing the Tal2a effector, the designer TAL effector dT280 which targets a sequence overlapping the predicted Tal2a binding sequence in UCH, or the Tal11b effector. Results provide insight into the genes differentially regulated in a Tal2a- and dT280-specific manner. Overall design: Examination of mRNA levels in Oryza sativa L. ssp. japonica cv. Nipponbare leaves at 48 hours after inoculation. Each leaf was considered a separate biological replicate.
Sample: PXO99A with dTal280 replicate 1
SAMN03487371 • SRS911699 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted with the Qiagen RNeasy kit and manufacturer's protocol. These libraries were prepped with the Illumina TruSeq RNA Sample Prep v2 kit
Experiment attributes:
GEO Accession: GSM1659655
Links:
External link:
Runs: 1 run, 29.6M spots, 3G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
SRR197651429,595,8133G2.1Gb2015-05-01

ID:
1448368

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