Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Different groups of HepG2 cell lines were flash frozen on dry ice, and RNA was harvested using Trizol reagent.We used 2 ug total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard nova6000 Illumina PE150 protocol.