RNAseq of female Lymantria dispar dispar pupa, CT strain, day 3 - SRA

SRX9152880: RNAseq of female Lymantria dispar dispar pupa, CT strain, day 3
1 ION_TORRENT (Ion Torrent Proton) run: 11.6M spots, 1.4G bases, 1Gb downloads

Design: Samples were homogenized in Trizol reagent (Ambion, Life Technologies; 4 to 7 mL depending on the size of the pupa) using 15 mL disposable tissue grinders (VWR, Cat.47732-446). For each sample, 500 L of the homogenate was transferred to a 1.5 mL microfuge tube, to which another 500 L of Trizol was added. The tubes were then centrifuged for 4 min at 17,000 x g to pellet debris. 400 L of the supernatant was subsequently used for RNA extraction using the Direct-zol RNA miniprep kit (Zymo Research). RNA was eluted using 50 L of water, quantified using a NanoDrop ND1000 spectrophotometer (Thermo Fisher Scientific Inc.), and assessed for quality using a 2100 Bioanalyzer system (Agilent). RNASeq libraries and Ion Proton sequencing were performed at the Genomic Analysis Platform of the Institute of Integrative and Systems Biology (Laval University, Quebec, QC, Canada) using the NEBNext Ultra II directional RNA library prep kit (New England BioLabs) with the NEBNext Poly (A) mRNA magnetic isolation module. Ion Proton sequencing was performed on an Ion Chef with a P1 chip according to manufacturer's instructions.

Submitted by: Natural Resources Canada

Study: Gypsy moth gene expression during female pupal development

PRJNA664407 • SRP283905 • All experiments • All runs

show Abstracthide Abstract

This project aims to compare the gene expression of female pupae from Asian (flight capable) and European (flight incapable) populations.

Sample: Gypsy Moth Strain CT, Day 3, Replicate 3

SAMN16213545 • SRS7392856 • All experiments • All runs

Organism: Lymantria dispar dispar

Library:

Name: Cusson_F1

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: SINGLE

Runs: 1 run, 11.6M spots, 1.4G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR12672688	11,579,933	1.4G	1Gb	2024-06-06

ID:: 11925464

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity