GSM4772443: GRA415A262; Homo sapiens; RNA-Seq - SRA

SRX9093020: GSM4772443: GRA415A262; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 28.2M spots, 5.7G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)

Study: Blood transcriptomics for diagnosis, risk, and treatment monitoring in tuberculosis reveal the evolution and resolution of TB disease: Does one signature capture all?

PRJNA662344 • SRP281425 • All experiments • All runs

show Abstracthide Abstract

Characterization of Tuberculosis (TB) progression, diagnosis and treatment response with blood transcriptional RNA-seq analyses. Focus and underlying host immunological response and identification of new signatures for TB early diagnosis and treatment monitoring response. Overall design: Blood transcriptomics RNA-seq dataset that includes blood RNA samples collected from TB progressors at different time points before they progress to active TB disease and at time of diagnosis before anti-TB treatment (ATT), and also samples from active TB patients from diagnosis before any anti-TB treatmentATT and followed during treatment at regular time points up to one year after treatment initiation.

Sample: GRA415A262

SAMN16081832 • SRS7338789 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was isolated, from blood from tuberculosis progressors and Treatment reponse cohorts, and IGRA-ve healthy controls simultaneously, from 1ml whole blood using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit . Globin RNA was depleted from the total RNA (1.5–2 μg) using the human GLOBINclear kit. Samples (200 ng) with an RQS > 6 were used to prepare a cDNA library using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). The tagged libraries were sized and quantitated in duplicate (Agilent TapeStation system) using D1000 ScreenTape and reagents (Agilent), normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired-end reads (100 bp) per sample

Experiment attributes:

GEO Accession: GSM4772443

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 28.2M spots, 5.7G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR12609934	28,150,582	5.7G	1.7Gb	2021-09-05

ID:: 11815389

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