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SRX8840965: GSM4699101: BN207 low potassium rep2; Triticum aestivum; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 27.3M spots, 8.1G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)
Study: Multi-omics Analyses Reveal Molecular Mechanisms of Potassium Deprivation Adaption in Wheat (Triticum aestivum L.)
show Abstracthide Abstract
This study evaluated the level of genetic variation among 543 wheat associations differing in K-deficiency tolerance at seedling and adult plant stages. Two of the 543 wheat associations, i.e. KN9204 and BN207, were identified as extreme K-deficiency tolerant and sensitive cultivars, respectively. We further conducted transcriptomic and metabolomics analyses using the roots of KN9204 and BN207 under normal or K-deficient conditions.Integrated analysis of gene expression and metabolite profiles revealed that dramatically more genes including those involved in ion homeostasis, cellular reactive oxygen species (ROS) homeostasis and glutamine synthetase pathways were induced in KN9204 as compared with BN207 under K-deficient conditions, which might indicate their unique roles in regulating plant K-starvation tolerance. These findings provided a better understanding of molecular responses of root adaptive strategies to K deprivation in wheat. Overall design: Root mRNA profiles of Kenong 9204 and Bainong 207 treated with normal potassium and low potassium for 14 days
Sample: BN207 low potassium rep2
SAMN15662669 • SRS7104174 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Aglient 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Poly(A) RNA was purified from total RNA using poly-T oligo-attached magnetic beads by two rounds of purification. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA).
Experiment attributes:
GEO Accession: GSM4699101
Links:
Runs: 1 run, 27.3M spots, 8.1G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR1234125727,330,8758.1G2.4Gb2020-07-29

ID:
11497554

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