GSM4672499: WT-1; Bacillus subtilis; RNA-Seq - SRA

SRX8740592: GSM4672499: WT-1; Bacillus subtilis; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 18.5M spots, 2.8G bases, 1Gb downloads

Submitted by: NCBI (GEO)

Study: NusG is an intrinsic transcription termination factor that stimulates motility and cooperatively coordinates global gene expression with NusA

PRJNA646522 • SRP272134 • All experiments • All runs

show Abstracthide Abstract

In this work we conducted Term-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis. Using this approach, we found that NusG is an intrinsic termination factor that works single and cooperatively with NusA. Overall design: Term-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis followed by in vitro and physiological follow-up

Sample: WT-1

SAMN15545441 • SRS7013119 • All experiments • All runs

Organism: Bacillus subtilis

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted from collected cell paste via RNeasy mini-kit (Qiagen, cat # 74104) total RNA was dephosphorylated using CIP (NEB, discontinued), rRNA was depleted via ribo-zero Gram-positive rRNA depletion kits (Illumina, discontinued), remaining RNA was ligated to custom unique 2', 3' dideoxy RNA oligonucleotide (IDT), Illumina sequencing libraries were generated from ligation products using TruSeq Stranded mRNA kit (Illumina, cat # 20020594) Term-seq

Experiment attributes:

GEO Accession: GSM4672499

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 18.5M spots, 2.8G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR12232093	18,510,210	2.8G	1Gb	2021-04-19

ID:: 11368412

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity