Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: 5-month old Nematostella vectensispolyps were dissected into 4 separate tissues: tentacles, mesenteries, pharynx, bodywall. Tissue pieces were treated with undiluted TrypLETM Select (Thermo Fisher, A1217701). For 1 hour the tissues were allowed to disintegrate in the enzyme with gentle agitation on a shaker, then dissociation into single-cell suspensions was completed with occasional pipetting over the course of another 1.5 hrs. Cell viability and counts were assayed with a Cellometer X2 (Nexcelom), and suspensions were diluted to 1000 – 1700 cells/uL and loaded into a 10x Genomics single cell platform using Vs.2 reagents. 10X Genomics Chromium v2 chemistry