Instrument: Illumina HiSeq 4000
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Dictyostelium were physically dissociated and washed twice with PBS with 0.04% BSA and lysed with 10 mM Tris-HCL (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.5% Tween 20, 0.5% NP-50, 0.05% digitonin (Promega G944A), 1% BSA, and 0.5% cellulase on ice or room temperature for 30 minutes to fully lyse the cells. Validation of equivalent ATACseq lysine of different stages was performed by trypan blue staining and optimized by real-time PCR detection of ribosomal RNA release. Nuclei were transposed using the Nextera DNA Library Prep Kit. Transposed libraries were amplified for 11 cycles and size selected for fragments ranging 200-1000 bp. ATAC-seq experiments were sequenced to a minimum depth of 25 million reads/sample.