GSM1597944: Bt_IgActl_rep2; Bacteroides thetaiotaomicron; RNA-Seq - SRA

SRX860747: GSM1597944: Bt_IgActl_rep2; Bacteroides thetaiotaomicron; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 5.4M spots, 225.4M bases, 129.6Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Characterizing the interactions between a naturally-primed IgA and its conserved Bacteroides thetaiotaomicron species-specific epitope in gnotobiotic mice [RNA-Seq]

PRJNA274030 • SRP052979 • All experiments • All runs

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We report the mid-log phase transcriptional profile of the human gut symbiont Bacteroides thetaiotaomicron, grown in the presence of mouse monoclonal IgAs with species- and strain-level specificity (mAb 260.8 and mAb 225.4, respectively). Overall design: mRNA expression profiling of the type strain of Bacteroides thetaiotaomicron (NC_004663) grown in vitro in the presence of either (i) PBS (carrier control), (ii) mAb 225.4 alone, (iii) mAb 260.8 alone, (iv) an equal combination of mAbs 225.4 and 260.8, or (v) a nonbinding control mouse mAb IgA (JC63.1)

Sample: Bt_IgActl_rep2

SAMN03322496 • SRS832322 • All experiments • All runs

Organism: Bacteroides thetaiotaomicron

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were extracted using acidic phenol:chlorophorm:isoamyl alcohol (125:24:1, pH 4.5). rRNA was removed using Ribo-zero. Illumina adapters were ligated to the DNA with T4 DNA ligase and cDNAs were size-selected by 2% agarose gel electrophoresis. PCR of the adapter-ligated product was performed , and samples were pooled in equal proportion.

Experiment attributes:

GEO Accession: GSM1597944

Links:

External link: GEO Sample GSM1597944

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.4M spots, 225.4M bases, 129.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR1781592	5,365,912	225.4M	129.6Mb	2015-06-29

ID:: 1233554

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